pneumacult-ali maintenance medium (STEMCELL Technologies Inc)
90
Structured Review
STEMCELL Technologies Inc
pneumacult-ali maintenance medium
Pneumacult Ali Maintenance Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pneumacult-ali+maintenance+medium/pneumacult+ali+medium/pmc07832566__mmc2-230-17-20
Average 90 stars, based on 1 article reviews
Pneumacult Ali Maintenance Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pneumacult-ali+maintenance+medium/pneumacult+ali+medium/pmc07832566__mmc2-230-17-20
Average 90 stars, based on 1 article reviews
pneumacult-ali maintenance medium - by Bioz Stars,
2026-10
90/100 stars
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Isolation:Article Title: Effect of Electronic Cigarette Vapour Exposure on Ca 2+ - and cAMP-Dependent Ion Transport in Human Airway Epithelial Cells. Article Snippet: Cells were air-exposed by removing the apical medium, and the basolateral medium was replaced with Article Title: Woodsmoke and diesel exhaust: Distinct transcriptomic profiles in the human airway epithelium. Article Snippet: HBECs were then incubated in submerged cultures for 4 days in Article Title: Loss of apolipoprotein E contributes to inflammatory macrophage activation and ferroptosis in NSAID-exacerbated respiratory disease Article Snippet: Supernatants were harvested for ELISA or liquid-64 chromatography tandem-mass spectrometry (LC-MS/MS, in 50% methanol [v/v], Applichem) 65 and cells were lysed in RLT buffer with 0.1 % -mercaptoethanol (Quiagen, Hilden, Germany) 66 or pelleted. Article Title: Transcriptomic Analysis of Air–Liquid Interface Culture in Human Lung Organoids Reveals Regulators of Epithelial Differentiation Article Snippet: For the ALI culture, the cells were seeded at a density of 5 × 10 4 cells per well in a 24-well transwell plate coated with 2% growth factor-reduced Matrigel and cultured in a PneumaCult Ex-plus medium until full confluency was reached, after which the old medium was removed from the apical chambers and the bottom chamber medium was exchanged for a Article Title: MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells Article Snippet: Isolated CPM+ cells were cultured at a density of 9.43 105 cells/cm on a culture plate with Article Title: Loss of apolipoprotein E contributes to inflammatory macrophage activation and ferroptosis in NSAID-exacerbated respiratory disease Article Snippet: 56 aMDM were differentiated in medium supplemented with 10 ng/mL rhGM-CSF (Miltenyi) and 57 2 ng/mL rhTGFβ1 (PeproTech, Hamburg, Germany) and MDM in medium containing 20 ng/mL 58 rhM-CSF (Miltenyi). Article Title: Macrolide resistance through uL4 and uL22 ribosomal mutations in Pseudomonas aeruginosa Article Snippet: Subsequently, ALI conditions were initiated by aspirating the media from the apical chamber and replacing the basolateral chamber media with Pneumacult-ALI maintenance medium (STEMCELL Technologies) supplemented with 480 ng/mL hydrocortisone, 4 μg/mL Article Title: A Ventilated Three-Dimensional Artificial Lung System for Human Inhalation Exposure Studies. Article Snippet: After 2–3 days, when confluence was reached, the media from both the basal and apical chambers were removed, and 1 mL of Cell Culture:Article Title: Effect of Electronic Cigarette Vapour Exposure on Ca 2+ - and cAMP-Dependent Ion Transport in Human Airway Epithelial Cells. Article Snippet: Cells were air-exposed by removing the apical medium, and the basolateral medium was replaced with Article Title: Woodsmoke and diesel exhaust: Distinct transcriptomic profiles in the human airway epithelium. Article Snippet: HBECs were then incubated in submerged cultures for 4 days in Article Title: Loss of apolipoprotein E contributes to inflammatory macrophage activation and ferroptosis in NSAID-exacerbated respiratory disease Article Snippet: Supernatants were harvested for ELISA or liquid-64 chromatography tandem-mass spectrometry (LC-MS/MS, in 50% methanol [v/v], Applichem) 65 and cells were lysed in RLT buffer with 0.1 % -mercaptoethanol (Quiagen, Hilden, Germany) 66 or pelleted. Article Title: Transcriptomic Analysis of Air–Liquid Interface Culture in Human Lung Organoids Reveals Regulators of Epithelial Differentiation Article Snippet: For the ALI culture, the cells were seeded at a density of 5 × 10 4 cells per well in a 24-well transwell plate coated with 2% growth factor-reduced Matrigel and cultured in a PneumaCult Ex-plus medium until full confluency was reached, after which the old medium was removed from the apical chambers and the bottom chamber medium was exchanged for a Article Title: MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells Article Snippet: Isolated CPM+ cells were cultured at a density of 9.43 105 cells/cm on a culture plate with Article Title: Loss of apolipoprotein E contributes to inflammatory macrophage activation and ferroptosis in NSAID-exacerbated respiratory disease Article Snippet: 56 aMDM were differentiated in medium supplemented with 10 ng/mL rhGM-CSF (Miltenyi) and 57 2 ng/mL rhTGFβ1 (PeproTech, Hamburg, Germany) and MDM in medium containing 20 ng/mL 58 rhM-CSF (Miltenyi). Article Title: Macrolide resistance through uL4 and uL22 ribosomal mutations in Pseudomonas aeruginosa Article Snippet: Subsequently, ALI conditions were initiated by aspirating the media from the apical chamber and replacing the basolateral chamber media with Pneumacult-ALI maintenance medium (STEMCELL Technologies) supplemented with 480 ng/mL hydrocortisone, 4 μg/mL Article Title: A Ventilated Three-Dimensional Artificial Lung System for Human Inhalation Exposure Studies. Article Snippet: After 2–3 days, when confluence was reached, the media from both the basal and apical chambers were removed, and 1 mL of |